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Features of biological effects in cell culture in the combined exposure to ionizing radiation and copper ions
D. D. Hapeienko1, H. I. Lavrenchuk1, V. S. Asmolkova2, V. N. Oksamytnyi1
1State Institution “National Research Center for Radiation Medicine of the National Academy of MedicalSciences of Ukraine”, Melnykov str., 53, Kyiv, 04050, Ukraine
2A.V. Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Leontovych str.,9, Kyiv, 01601, Ukraine
The objective of the work was to research the features of mono- and combined impact of ionizing radiation and copper salts on cell viability in vitro.
Materials and methods. The studies were performed on a passaged line L929 cell culture. Copper ions of different concentrations were added to the culture medium 1 hour after exposure to irradiation at doses of 0.5; 5.0 and 10.0 Gy. There were evaluated the structure of the cell populations, the proliferative and mitotic activity and apoptosis.
Results and conclusions. After incubation of the cells with copper ions of various concentrations there were seen both the activation and inhibition of proliferative and mitotic activity. Cell survival and mitotic activity were decreased in exposure to increasing doses of radiation but the polykaryocyte index was increased. After the combined in vitro exposure to ionizing radiation and copper ions there were seen morphofunctional changes of cell properties, which were not in direct proportional dependence on the dose or the copper ions concentration.
Key words: heavy metals, ionizing radiation, cell culture, survival, proliferation, apoptosis.
Problems of radiation medicine and radiobiology. 2013;18:398-406.
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