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Formation peculiarities of radiation-induced aberrations of chromosomes in human cells under the modifying influence of chemical agents (comparative aspects)
E. A. Dyomina, O. P. Pylypchuk
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine,45, Vasylkivska str., Kyiv, 03022
Objective. The study objective was to determine and provide a comparative analysis of frequency and spectrum of the induced aberrations of chromosomes in culture of the human peripheral blood lymphocytes under the combined impact of radiation, co-mutagen, and chemical mutagen.
Methods. Culture of human peripheral blood lymphocytes and cytogenetic methods have been used.
Results. A co-mutagenic effect of the drug verapamil was established under the testing γ-irradiation of human peripheral blood lymphocytes in the dose range of 0.3–2.0 Gy at the expense of increased frequency of chromosomal aberrations (dicentrics). The combined effect of γ-irradiation and S-Nitrosoglutathione is directed on the induction and storage of chemical markers of exposure – the chromatid-type aberrations.
Conclusion. A co-mutagenic effect of verapamil under the low-dose γ-irradiation as a 2-fold increase of the chromosome-type aberrations (radiation markers) incidence was revealed at a chromosomal level in human peripheral blood lymphocytes. Phenomenon of synergism of low-dose γ-irradiation and mutagen S-Nitrosoglutathione as a ~3-fold increased frequency of chromatid-type aberrations (chemical markers) was detected compared to the sole radiation effect.
Key words: co-mutagenesis, radiation, verapamil, S-Nitrosoglutathione, radiation-induced chromosomal aberrations, human somatic cells.
Problems of radiation medicine and radiobiology. 2013;18:330–337.
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