Problems of Radiation Medicine and Radiobiology

Nitric oxide and lipid peroxidation in children-residents of the contaminated territories

Ye. Stepanova, I. Kolpakov, V. Kondrashova, O. Litvinets
State Institution “National Research Center for Radiation Medicine of the National Academy of MedicalSciences of Ukraine”, Melnykov str., 53, Kyiv, 04050, Ukraine

Objective. The nitrogen oxide system assessment by the nitrogen oxide stable metabolites assay both with indices of lipid peroxidation and antioxidant protection of children residing on contaminated territories.
Materials and methods. Studies were conducted in children, born and residing in areas of contamination. Assay of nitrogenous species (NO2- and NO3-) was performed using a standard method with Griss-reagent. Biochemical assay of the malonic dyaldehide, catalase, and ceruloplasmin was applied to reveal the signs of oxidative stress.
Results and conclusions. The assay of nitric oxide metabolites in the blood serum of children-residents of contaminated territories revealed a reduction of nitrite and no significant changes in the content of both nitrate and total NO metabolites. There was an activation of the intracellular antioxidant catalase with the absence of significant changes in the basic serum ceruloplasmin antioxidant. All that was not accompanied with an increase of serum concentration of malonic dyaldehide i.e. the ТBA-active product of lipid peroxidation being indicative to the adequacy of compensatory capacity of antioxidant defense.

Key words: children, Chornobyl accident, contaminated territories, nitric oxide, lipid peroxidation.

Problems of radiation medicine and radiobiology. 2013;18:261–269.