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EXPRESSION OF GENE-REGULATORS OF APOPTOSIS AND CELL CYCLE IN PERIPHERAL BLOOD LYMPHOCYTES IN COGNITIVE IMPAIRMENT OF CHORNOBYL CLEAN-UP WORKERS
[Article in Ukrainian]
I. Ilyenko*, D. Bazyka, S. Chumak, K. Loganovsky
State Institution “National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine”, 53, Melnykov str., Kyiv, 04050, Ukraine
*Corresponding author: IrynaIlyenko, e-mail: ilyenko@ukr.net
Aim — to determine the characteristics and nature of changes in expression of gene-regulators of apoptosis and cell cycle progression in peripheral blood lymphocytes of Chornobyl clean-up workers with cognitive function impairment. The study of relative quantification of expression of genes TP53, TP53I3, FASLG, BAX, CCND1 was performed by polymerase chain reaction in PCR real time using TagMan technologies. The study involved 120 subjects and different stages of cognitive impairment, including the Chornobyl clean-up workers 1986–87. Subjects with mild cognitive disorders were characterized by an increase in the expression of proapoptotic genes TP53 and TP53I3. Dependency of gene expression and TP53 TP53I3 of the dose is not established. Documented reduction in average RQ of BAX was demonstrated in groups of Chornobyl clean-up workers of 1986–87 with mild cognitive deficit as well as in patients with vascular dementia. A negative correlation was shown between the level of BAX gene expression and radiation dose in the group of Chernobyl clean-up workers 1986–87 with mild cognitive deficits, and a dose-related overexpression of CCND1 in the peripheral blood lymphocytes of patients with cognitive deficit. The highest CCND1 gene RQ figures were shown in patients with dementia in the late period after irradiation.
Key words: ionising radiation, gene expression, cognitive functions, apoptosis, Chornobyl.
Probl. Radiac. Med. Radiobiol. 2012. Issue 17. P.163–175.
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