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REGULATORY T-CELLS SUBPOPULATIONS AND APOPTOSIS OF PERIPHERAL BLOOD LYMPHOCYTES IN PERSONS EXPOSED IN UTERO AS A RESULT OF THE CHORNOBYL ACCIDENT, WITH VEGETOVASCULAR DYSTONIA IN REMOTE PERIOD
[Article in Russian]
N. V. Belyaeva*, N. A. Golyarnik, O. A. Beliaev, O. L. Maznichenko
State Institution “National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine”, Melnykov str., 53, Kyiv, 04050, Ukraine
*Corresponding author: Nadiia V. Belyaeva, e-mail: nbelnbel@mail.ru
The aim of the study was to reveal the pathogenetic significance of changes in the phenomenon apoptosis of peripheral blood (PB) lymphocytes and in status of subpopulations of regulatory T cells in persons exposed in utero as a result of the Chornobyl accident with vegetovascular dystonia (VVD) in remote period. Two groups of patients with VVD — prenatally exposed with doses to the brain from 0.022 mSv to 24.3 mSv (the main study group) and non-irradiated (the comparative study group) were included in the study; 48 persons in total who were born between April 26, 1986 to February 26, 1987. All patients carried out immunophenotyping and study of apoptosis of lymphocytes by CD95 expression and in Annexin V assay by flow cytometry. The set of identified changes in the structure of the subpopulation of CD4 T-helper and apoptosis of lymphocyte suggests the presence of immuno-inflammatory process in the CNS, allows us to assume that the decrease of natural regulatory T cells and disturbance of Fas-dependent apoptosis of lymphocytes in VVD are of pathogenetic significance. “Deficiency of negative immunoregulation” with an increased risk of autoimmune disorders can be considered among other mechanisms in the pathogenesis of VVD in patients who had been exposed in utero.
Key words: accident at Chornobyl, radiation-exposed in utero, apoptosis, regulatory T cells.
Probl. Radiac. Med. Radiobiol. 2012. Issue 17. P. 136–143.
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