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L. M. Zvarych, O. V. Lyaskivska, D. A. Bazyka, V. V. Panchenko
State Institution «National Research Center for Radiation Medicine, Hematology and Oncology of the
National Academy of Medical Sciences of Ukraine», 53 Yuriia Illienka St., Kyiv, 04050, Ukraine
CHANGES IN RELATIVE CYTOKINE LEVELS AND FUNCTIONAL STATUS OF AUTOPHAGY IN PERIPHERAL BLOOD LYMPHOCYTES IN THE REMOTE PERIOD AFTER IRRADIATION
Objective: to assess the relative levels of cytokines and the functional state of autophagy in peripheral blood lymphocytes of clean-up workers of the Chornobyl accident.
Materials and methods. A total of 42 male patients aged 51–77 (61,35 ± 5,21) years were examined. They included: the main group – 42 clean-up workers of the Chornobyl accident aged 56–63 (60,16 ± 1,73) years; and the control group – 11 persons aged 51–77 (64,72 ± 9,24) years, who did not participate in the liquidation of the consequences of the Chornobyl accident and did not live in radioactively contaminated areas. Analysis of relative levels
of cytokines, NF-κB p65, and autophagy parameters in peripheral blood (PB) lymphocytes was performed using flow
cytometry.
Results. The decrease in the relative levels of IL-1β, IL-2, and IFN-γ and an increase in IL-6, TNF-α, and TGF-β in the
PB lymphocytes of the clean-up workers of the Chornobyl accident were detected. Correlation analysis showed the
presence of a relationship between the levels of the studied cytokines. The relative level of NF-κB p65, the basal
level of the key autophagy protein LC3B and after the addition of chloroquine diphosphate in the PB lymphocytes
of the clean-up workers of the Chornobyl accident were similar to those in the control group. The decrease in the
capacity and responsiveness of the autophagic flux in the PB lymphocytes of the clean-up workers of the Chornobyl
accident was noted. Direct correlations were found between the basal level of the LC3B protein and IL-4 and IL-6 in
the PB lymphocytes of the clean-up workers of the Chornobyl accident.
Conclusions. According to preliminary data, the detected changes in the clean-up workers of the Chornobyl accident may contribute to the activation of pathological inflammatory processes and autoimmune reactions due to
impaired lymphocyte function and inflammatory reactions due to a shift in the balance between cytokines and positive regulation of their synthesis.
Key words: autophagy; LC3B, cytokines; lymphocytes; inflammation; ionizing radiation; Chornobyl accident.
Problems of Radiation Medicine and Radiobiology.
2025;30:398-413. doi: 10.33145/2304-8336-2025-30-398-413
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