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I. Z. Russu, N. M. Bilko
National University of Kyiv"Mohyla Academy, 2 Grygoriya Skovorody St., Kyiv, 04070, Ukraine
INFLUENCE OF BONE MARROW MESENCHYMAL STROMAL CELLS ON THE FUNCTIONING OF HEMATOPOIETIC CELLS UNDER SUBLETHAL IRRADIATION
Objective: investigation of the influence of mesenchymal stromal cells on the functioning of hematopoietic cells
of Balb/C mice after the exposure to ionizing radiation at a sublethal dose in cell culture in vitro and in vivo.
Methods. To study the influence of stromal microenvironment, a co-cultivation model of hematopoietic cells of irradiated animals and mesenchymal cells forming feeder layers was used in the in vitro culture. Using the trypan blue
dye, the number of hematopoietic cells with cytoplasmic membrane damage was assessed and the level of cell death
was determined. Cell cultivation in the diffusion chambers culture in vivo allowed assessing the proliferative potential of bone marrow hematopoietic stem cells of irradiated animals.
Results. Investigation of the interaction between bone marrow stromal and hematopoietic cells of Balb/C mice
allowed determining the modifying influence of the stroma on the level of progenitor cells’ interphase and reproductive death due to ionizing radiation action at a sublethal dose. In particular, after 2 days the percentage of dead
cells in suspension with direct contact with the stroma in vitro was reduced compared to hematopoietic cells where
such contact was absent: (40.5 ± 2.6) % vs (60.1 ± 2.8) %. The increase in cell aggregates number was also observed
in the cultures of irradiated progenitors, which had previously interacted with mesenchymal stromal cells in vitro
(25.2 ± 1.9 CFU), compared to the cells, which did not directly interact with the stroma (15.3 ± 2.8 CFU).
Conclusions. Co-cultivation with stromal cells have significantly reduced the level of radiation-induced death of
bone marrow hematopoietic cells and increased their colony-forming activity, however, this required the direct contact with stroma in the in vitro culture.
Key words: hematopoiesis; ionizing radiation; stroma; bone marrow; Balb/C mice; stem cells; cell culture
in vitro and in vivo.
Problems of Radiation Medicine and Radiobiology.
2025;30:273-283. doi: 10.33145/2304-8336-2025-30-273-283
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