|
I. Ye. Kolpakov, V. Ì. Zigalo, V. H. Kondrashova, V. A. Poznysh, L. O. Leonovych
State Institution «National Scientific Center for Radiation Medicine, Hematology and Oncology of the
National Academy of Medical Sciences of Ukraine», 53 Yuriia Illienka Str., Kyiv, 04050, Ukraine
SUPEROXIDE DISMUTASE (SOD2) GENE VAL16ALA POLYMORPHISM AND CHANGES IN THE VENTILATION LUNG CAPACITY OF CHILDREN – RESIDENTS OF RADIOACTIVELY CONTAMINATED TERRITORIES AND CHILDREN WHO HAVE BEEN AFFECTED BY STRESSFUL LIFE EVENTS DURING WARTIME
Objective: to determine the distribution of genotypes of genetic polymorphism of manganese superoxide dismutase
and to assess the ventilation lung capacity in children- residents of radioactively contaminated areas and children
exposed to stressful life events during the war period.
Materials and methods. The study involved school-age children – residents of radioactively contaminated areas
(RCA) and children exposed to stressful life events during wartime. All those examined had no clinical signs of respiratory pathology. Genotypes for the SOD2 Val16Ala genetic variant were determined using the polymerase chain
reaction (PCR) method. The study of the ventilation lung capacity was assessed by the method of computer spirometry based on the flow-volume loop analysis data.
Results and conclusions. When studying the genotypes and alleles of the polymorphic marker Superoxide
Dismutase 2 Gene Val16Alain children of group I and II, no significant differences were found between the frequency
distribution indicators of genotypes and alleles compared with the reference values of the those of the control
group, which consisted of practically healthy residents of the Middle East. Also, no significant differences in the frequency distribution of the C and T alleles of the polymorphic marker Superoxide Dismutase 2 Gene Val16Ala were
found in the children of both examined groups compared to other representatives of the Caucasian race (Lithuanians, Finns, Germans). Among children in groups I and II, there was a tendency toward a decrease in the frequency of occurrence of homozygotes with the CC genotype and an increase in the frequency of occurrence of heterozygotes with the CT genotype. Among children of both examined groups, in heterozygotes with the CT genotype of the
Superoxide Dismutase 2 Gene Val16Ala polymorphism, a tendency towards an increase in the frequency of bronchial
hyperreactivity was observed compared to CC homozygotes. Analysis of the variant allele frequency distribution of
SOD2 Val16Ala polymorphism in children of both examined groups determined that in the presence of bronchial hyperreactivity there was a tendency toward an increase in the prevalence of the T allele and a decrease in the prevalence
of the C allele.
Key words: children; radioactively contaminated areas; bronchial hyperreactivity; genetic polymorphism of manganese superoxide dismutase.
Problems of Radiation Medicine and Radiobiology.
2024;29:359-374. doi: 10.33145/2304-8336-2024-29-359-374
 full text
|
