O. V. Shemetun, O. A. Talan, O. B. Dibska
State Institution «National Scientific Center for Radiation Medicine, Hematology and Oncology of the
National Academy of Medical Sciences of Ukraine», 53 Yuriia Illienka Str., Kyiv, 04050, Ukraine
STUDY OF CHROMOSOME STABILITY OF HUMAN SOMATIC CELLS IN THE DEVELOPMENT OF THE SECONDARY RADIATION-INDUCED BYSTANDER EFFECT
Objective. To establish the level of chromosomal instability in human peripheral blood lymphocytes during the
development of secondary radiation-induced bystander effect.
Materials and methods. Human peripheral blood lymphocytes; culture of human non-small-cell lung cancer cell line
A549 (irradiated in vitro by 137Cs in a dose of 0.50 Gy/unirradiated). Cytogenetic analysis of uniformly colored chromosomes of human peripheral blood lymphocytes with determination of the frequency of all types of aberrations.
Results. The mean group frequency of chromosome aberrations (4.75 per 100 cells) in human peripheral blood lymphocytes co-cultivated with conditioned culture medium from bystander cells of human non-small cell lung cancer
cell lines A-549 exceeded the control (1.60 per 100 cells) (p < 0.001) due to an increase in the frequency of single
fragments from 1.20 to 3.95 per 100 cells (p < 0.001). The mean group frequency of chromosome aberrations and
chromatid-type aberrations in human peripheral blood lymphocytes during the development of primary (5.80 and
4.35 per 100 cells, respectively) and secondary (4.75 and 3.95 per 100 cells, respectively) radiation-induced bystander effect did not significantly differ from each other (p > 0.05).
Conclusions. Under the conditions of co-cultivation of human peripheral blood lymphocytes with conditioned culture medium from bystander cells of human non-small cell lung cancer A-549, a secondary radiation-induced bystander effect was registered, the cytogenetic manifestation of which was an increase in the level of chromosomal
instability due to the rise in the frequency of chromatid-type aberrations (single fragments). The levels of chromatid-type chromosome aberrations during the development of the primary and secondary radiation-induced
bystander effect did not differ significantly. The development of the secondary radiation-induced bystander effect
did not affect the induction of chromosomal-type aberrations.
Key words: radiation-induced bystander effect; human peripheral blood lymphocytes; chromosome aberrations;
human non-small cell lung cancer cell lines A-549.
Problems of Radiation Medicine and Radiobiology. 2024;29:228-242. doi: 10.33145/2304-8336-2024-29-228-242
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