National Academy of Medical Sciences of Ukraine
State Institution "The National Research Center for Radiation Medicine"


ISSN 2313-4607 (Online)
ISSN 2304-8336 (Print)

Problems of Radiation Medicine and Radiobiology

  
 

   

G. Y. Lavrenchuk, V. V. Talko, A. V. Chernyshov

State Institution źNational Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine╗, 53 Yuriia Illienka Street, Kyiv, 04050, Ukraine

EFFECTIVENESS OF THE PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT HUMAN CELLS ▓N A RESTING STATE

Objective: to investigate the structural and morphofunctional changes in test system of malignant (cell line A-549) human cells in a resting state exposed to X-rays in the presence of gadolinium-containing photon capture agent źDotavist╗ and optical light (red spectrum) in combination with źPhotolon╗ photosensitizer.
Methods. Passaged malignant human cell culture technology, X-ray and red light exposure, cytological and statistical methods.
Results. X-ray exposure at a dose of 10.0 Gy in the presence of photon capture agent źDotavist╗ (at a 100 μg/ml nutrient medium concentration) led to death of 75ľ83 % of malignant cells in a resting state on the 6ľ8th day of cultivation. Photodynamic exposure (630 nm wavelength red light) in the presence of źPhotolon╗ photosensitizer (200 μg/ml concentration) resulted in death of 69ľ73 % of malignant cells, respectively. Combination of the photon-capturing technology and photodynamic exposure resulted in death of 90 % of the malignant cells in a phase of steady-state growth on the 8th day of cultivation.
Conclusion. Combination of the photon capture technology (X-ray exposure with gadolinium-containing photon capture agent źDotavist╗ in cytotoxic concentration) and photodynamic exposure in the presence of źPhotolon╗ photosensitizer increased devitalization effectiveness of human non-small cell lung cancer cells (A-549 cell line) being in a steady-state growth phase up to 90 %. Ten percent of cells resistant to the applied technologies retained their proliferative potential, evident as changes in their morphology, genotype and adhesiveness during further cultivation.
Key words: culture of human malignant cells, X-ray irradiation, photon capture agent, red light, photosensitizer.

Problems of Radiation Medicine and Radiobiology.
2023;28:206-215. doi: 10.33145/2304-8336-2023-28-206-215

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