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G. Y. Lavrenchuk, V. V. Talko, A. V. Chernyshov
State Institution «National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine», 53 Yuriia Illienka Street, Kyiv, 04050, Ukraine
EFFECTIVENESS OF THE PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT HUMAN CELLS ²N A RESTING STATE
Objective: to investigate the structural and morphofunctional changes in test system of malignant (cell line A-549)
human cells in a resting state exposed to X-rays in the presence of gadolinium-containing photon capture agent
«Dotavist» and optical light (red spectrum) in combination with «Photolon» photosensitizer.
Methods. Passaged malignant human cell culture technology, X-ray and red light exposure, cytological and statistical methods.
Results. X-ray exposure at a dose of 10.0 Gy in the presence of photon capture agent «Dotavist» (at a 100 μg/ml
nutrient medium concentration) led to death of 75–83 % of malignant cells in a resting state on the 6–8th day of
cultivation. Photodynamic exposure (630 nm wavelength red light) in the presence of «Photolon» photosensitizer
(200 μg/ml concentration) resulted in death of 69–73 % of malignant cells, respectively. Combination of the photon-capturing technology and photodynamic exposure resulted in death of 90 % of the malignant cells in a phase
of steady-state growth on the 8th day of cultivation.
Conclusion. Combination of the photon capture technology (X-ray exposure with gadolinium-containing photon
capture agent «Dotavist» in cytotoxic concentration) and photodynamic exposure in the presence of «Photolon»
photosensitizer increased devitalization effectiveness of human non-small cell lung cancer cells (A-549 cell line)
being in a steady-state growth phase up to 90 %. Ten percent of cells resistant to the applied technologies retained
their proliferative potential, evident as changes in their morphology, genotype and adhesiveness during further cultivation.
Key words: culture of human malignant cells, X-ray irradiation, photon capture agent, red light, photosensitizer.
Problems of Radiation Medicine and Radiobiology.
2023;28:206-215. doi: 10.33145/2304-8336-2023-28-206-215
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