Problems of Radiation Medicine and Radiobiology

Ye. I. Stepanova, I. Ye. Kolpakov, V .Yu. Vdovenko, V. М. Zigalo, V. H. Kondrashova,
О. S. Leonovich

State Institution «National Research Center for Radiation Medicine of the National Academy of Medical
Sciences of Ukraine», 53 Yuriia Illienka St., Kyiv, 04050, Ukraine

MOLECULAR GENETIC ASPECTS OF BRONCHIAL HYPERREACTIVITY IN CHILDREN – RESIDENTS OF RADIOACTIVELY CONTAMINATED AREAS

Objective. to determine the relationship between polymorphisms of glutathione S-transferase gene family and bronchial hyperreactivity in children living in radioactively contaminated areas.
Materials and methods. School age children-residents of radioactively contaminated areas (RCA), without clinical signs of respiratory pathology were examined. Molecular genetic studies were carried out by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) for further analysis. The GSTT1, GSTM1 gene deletion polymorphism was investigated using multiplex PCR. PCR and PCR-RFLP analyses were performed in the study of the GSTP1 gene A313G polymorphism. The ventilation lung capacity was examined by the pneumotachographic method according to the analysis of «the flow–volume» loop. The pharmacologic inhalation test with bronchodilator drug, affecting the β₂-adrenergic lung receptors was used to detect the early changes in the ventilation lung capacity – the bronchial hyperreactivity (latent and nonlatent bronchospasm).
Results. Molecular genetic studies showed that the GSTM1 gene deletion genotype and the GSTP1 gene A313G polymorphism were found significantly more often in the subgroup of children with bronchial hyperreactivity living in RCA than in children without bronchial hyperreactivity and children of the control group. The frequency of GSTT1 deletion polymorphism did not have a statistically significant difference in all subgroups.
Conclusions. The GSTM1 gene deletion polymorphism and the GSTP1 gene A313G genotype may be a risk factor for developing bronchial hyperreactivity in children living under adverse environmental conditions, including radioactively contaminated areas.
Key words: children, radioactively contaminated areas, bronchial hyperreactivity, glutathione-S-transferase gene polymorphisms.

Problems of Radiation Medicine and Radiobiology.
2020;25:531-542. doi: 10.33145/2304-8336-2020-25-531-542

full text

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