Problems of Radiation Medicine and Radiobiology

Ye. I. Stepanova, I. Ye. Kolpakov, V. M. Zyhalo, V. Yu. Vdovenko, V. V. Vasylenko, O. M. Leonovych

State Institution «National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine», 53 Melnykova str., Kyiv, 04050, Ukraine

FEATURES OF NITRIC OXIDE METABOLISM AND RISK OF DEVELOPING ENDOTHELIAL DYSFUNCTION IN CHILDREN WITH e-NOS GENE 4a/4b POLYMORPHISM UNDER LONG/TERM ENTERING ¹³⁷Cs TO BODY

Objective. To determine the features of the nitrogen oxide metabolism and risk of developing endothelial dysfunction in children with e-NOS 4a/4b gene polymorphism, who live under prolonged enter ¹³⁷Cs to the body.
Materials and methods. There were examined 117 children-residents of radioactively contaminated territories and 50 children of control group. The level of stable metabolites was defined in blood serum (NO^2- and NO^3-). The thermographic method was used to register the endothelium dependent reaction of the vascular bed to changes in the blood supply. The ventilation capacity of the lungs was evaluated using this method of pneumotachography. Polymorphism in intron 4 of the gene e-NOS was studied by the method of polymerase chain reaction. The content of ¹³⁷Cs in the body of children was determined using a human radiation counter Skrynner M-3.
Results and conclusions. In children-residents of radioactively contaminated territories with genotype 4a/4b comparing to children who had genotype 4b/4b, the decrease in the nitric content of in the blood serum, the increase in the thermographic index of the recovery period of blood circulation to the baseline level after occlusion test were noted, that is indicative of the decreased NO-synthase active of vascular endothelium in the carriers of the minor allele a in the 4th intron of gene eNOS (genotype 4a/4b), and is a risk factor for development of endothelial dysfunction. It was proved a decrease in the index of lung tissue elasticity and stretchability – FVC / NFVC of the lungs comparing to children with genotype 4b/4b, there was a reduction of integral index of respiratory tract permeability – FEV₁/NFEV₁. The inverse correlation dependence between the presence of allele a in the genotype and the values of FVC/NFVC of the lungs (r = &0.259; p <0.05) and FEV₁/NFEV₁ (r = &0.2267; p <0.05) was found. Signs of bronchospasm were found in the carriers of the allele a in 1.5 times more often than in children-carriers of homozygotes from allele b.
Key words: children, radioactive cesium, nitric oxide, eNOS 4a/4b gene polymorphisms.

Problems of radiation medicine and radiobiology.
2018;23:462_470. doi: 10.33145/2304-8336-2018-23-462-470.

full text

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