Problems of Radiation Medicine and Radiobiology

Ye. I. Stepanova, I. Ye.Kolpakov, V. M. Zygalo, V. Yu. Vdovenko, V. H. Kondrashova,
O. S. Leonovich, V. V. Kryzhanivska

State Institution «National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine», Melnykova str., 53, Kyiv, 04050, Ukraine

Experience of correcting endothelial dysfunction in children-residents of radioactively contaminated areas by nitric oxide potential donator citrulline

Objective: to determine the effectiveness of citrulline use for correcting endothelial dysfunction in children-residents of radioactively contaminated areas.
Materials and methods. A group of children-residents of radioactively contaminated areas with the presence of clinical and paraclinical signs of endothelial dysfunction was selected to assess the  effectiveness of correcting endothelial dysfunction by the usage of NO potential donator – citrulline according to the data of selective screening. There were determined the biochemical parameters of the content of NO stable metabolites, L-arginine, lipid peroxidation, antioxidant enzymes in the blood serum; the indices of cellular and humoral immunity; the instrumental indices of vascular endothelium-dependent reaction on occlusion test, the lung ventilation capacity, the bioelectric activity of the myocardium, the autonomic regulation of the cardiovascular system. Examined children were received a course of citrulline malate.
Results and conclusions. An increased content of serum L-arginine, nitrite, and amounts of NO metabolites was established in children with endothelial dysfunction who were received a course of citrulline malate. Bronchospasm elimination was noted in the significant part of examined patients after the drug use. Decreased recovery period and increased period of hypercompensation for thermographic circulation index in the test with post occlusion reactive hyperemia were detected by an evaluation of indicators for vascular endothelium dependent vasodilatation using thermographic method indicating an increased endothelial vasomotor capacity. There was tendency to improve the processes of autonomic regulation of the heart rhythm and repolarization of the heart muscle. The antioxidant effect of used citrulline malate course was determined as: decreased content of serum LPO end products that react with thiobarbituric acid under elevated activity of antioxidant – catalase. An increase in the percentage of T lymphocyte, normalization of their subpopulation composition was noted in dynamics of citrulline malate application.
Key words: children, radioactively contaminated territories, endothelial dysfunction, correction, citrulline.

Problems of radiation medicine and radiobiology. 2017;22:463–475.