Problems of Radiation Medicine and Radiobiology

V. M. Shkarupa¹, S. V. Klymenko², V. V. Talko²

¹ National Pirogov Memorial Medical University, 56 Pyrohova str., Vinnytsya, 21018, Ukraine
² State Institution «National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine», Melnykov str., 53, Kyiv, 04050, Ukraine

Modification of radiation-induced chromosome damage in Allium-test by sodium humate

Objective. Cytogenetic analysis of the effects of sodium humate in induced g-irradiation mutagenesis in Allium-test.
Materials and methods. Analysis of the root meristem cells of Allium cepa L. seeds carried by ana-telophase. Inves- tigated the effect of sodium humate (100 mg/l) on cytogenetic effects g-irradiation (137Cs) at doses of 5, 10 and 20 Gy.
Results. Antimutagenic effect of sodium humate was revealed, what allows to consider it as potential therapeutic modifier of radiation damage. Antimutagenic effect of sodium humate increased with increasing irradiation dose. Spotted differential activity of the drug with respect to various types of aberrations are most effectively reducing the frequency of radiation markers mutagenesis – chromosomal aberrations manifested in lower efficiency of long term survivors against potential changes of chromosomes compared with short-lived.
Conclusions. Revealed different  mechanisms for implementing antimutagenic properties of  sodium humate in con- dition of g-irradiation-induced mutagenesis in Allium-test.
Key words: antimutagenesis, sodium humate, g-irradiation, Allium-test.

Problems of radiation medicine and radiobiology. 2016;21:159–171.