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M. À. Pilinska1, D. À. Êurinnyi1, S. R. Rushkovsky2, Î. B. Dybska1
1State Institution «National Research Center for Radiation Medicine of the National Academy of Medical
Sciences of Ukraine», Melnykov str., 53, Kyiv, 04050, Ukraine
2Institute of Biology of Taras Shevchenko National University, Volodymyrska str., 64/13, Kyiv, 01601, Ukraine
Genoprotective properties of astaxanthin revealed by ionizing radiation exposure in vitro on human peripheral blood lymphocytes
Objective: to identify possible radioprotective properties of astaxanthin by means of cytogenetic criteria. Methods. Cultivation of peripheral blood lymphocytes from five apparently healthy volunteers; treatment of lym- phocytes’ cultures by astaxanthin in final concentrations 20 ?g/ml in Go phase of mitotic cycle, prior to ?- irradia- tion in vitro in a dose 1 Gy; cytogenetic analysis the uniformly stained slides of metaphase chromosomes. The elec- trophoresis of individual cells (Comet assay); visualization of results under fluorescent microscope; accounting the number of nucleoid the fourth grade that correspond to apoptosis of the cells.
Results. Established that astaxanthin in final concentration 20.0 ?g/ml exposed to the culture of human peripher- al blood lymphocytes in the early G0 phase of mitotic cycle leads to significant reduction of cytogenetic effects induced by gamma irradiation in vitro in dose 1.0 Gy (from 26.05 ± 1.81 to 9.08 ± 0.78 per 100 cells, respectively) and to significant increase the frequency of apoptotic cells at the 48 hour of cultivation (from (3.78 ± 0.24) to (8.26 ± 0.91) %, respectively).
Conclusions. The results obtained show the ability of astaxanthin to considerable weakening of radioinduced muta- genic effect in human peripheral blood lymphocytes, which testify its powerful radioprotective potential.
Key words: astaxanthin, culture of human peripheral blood lymphocytes, chromosome aberrations, apoptosis, radio- protective effect.
Problems of radiation medicine and radiobiology. 2016;21:141–148.
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