Problems of Radiation Medicine and Radiobiology

Russu I. Z., Rodionova N. K., Bilko D. I., Bilko N. M.

Pattern changes in quantitative and qualitative markers of hematopoietic stem cells during acute and chronic exposure to 90sr isotope in cell culture

The aim of our research was investigation of the hematopoietic system of laboratory rats under the influence of acute and chronic internal exposure to 90Sr isotope.
Materials and methods. To study the condition of stem cells and their immediate progenitors we implemented cell culture methodology in vivo in gel diffusion capsules with subsequent analysis of the colonies and clusters.
Results. On the basis of experiments it was established that long-term effects of incorporated 90Sr isotope leads to significant disturbances in the hematopoietic system and in particular, revealing changes in hematological parameters of irradiated animals such as the appearance of circulating progenitor cells in peripheral blood, reducing the colony-forming efficiency of the bone marrow derived progenitor cells, as well as quantitative and qualitative changes in the clones.
Conclusions. Indices confirm the connection of the detected effects in individuals exposed to ionizing radiation described in the earlier publications and can serve as basis for developing criteria for the formation of risk groups among people exposed to 90Sr.

Key words: hematopoiesis, progenitors, cell culture in diffusion chambers in vivo, internal irradiation, 90Sr.

Problems of radiation medicine and radiobiology. 2015;20:533-542.