National Academy of Medical Sciences of Ukraine
State Institution "The National Research Center for Radiation Medicine"

ISSN 2313-4607 (Online)
ISSN 2304-8336 (Print)

Problems of Radiation Medicine and Radiobiology



Nurishchenko N. E.

Effect of ultrasound on the interleukin content in blood of rats with experimental inflammation

The aim was to determine the effectiveness of anti-inflammatory action of ultrasound by the interleukin-6, interleukin-8 and tumor necrosis factor alpha content in the blood of rats with experimental inflammation.
Materials and methods: the study was performed using conventional models of inflammation - carrageenan-induced edema of the limbs of rats. Content interleukins IL-6 and IL-8 were determined by enzyme immunoassay (ELISA) according to a standard protocol Immunoassay kit IL-10, Biosourse (USA). The content of TNF-? was determined by cytotoxic effect on sensitive mouse fibroblast line L-929.
Results and discussion: it was shown that in the group of rats with carrageenan-induced edema contents of IL-6 increase in 2.8 times, IL-8 – in 5.6 times, and TNF-? – in 3 times compared to the control. Course influence of ultrasound contributed to reduction of the studied parameters. The content of IL-6 decreased in 1.7 times, IL-8 and TNF-? – in 1.6 times in comparison with inflammation.

Key words: ultrasound, inflammation, interleukin-6, interleukin-8, tumor necrosis factor alpha.

Problems of radiation medicine and radiobiology. 2015;20:526-532.

full text

Home | Editorial board | For Authors | Archive | Search
© 2013 Problems of Radiation Medicine