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Litvinchuk H. M., Lavrenchuk G. Y., Litvinchuk L. M., Asmolkova V. S., Boyko O. A.
Experimental evaluation of 2-merkaptobenztyazol radioprotective capacities on cell cultures test system
Objective. To study the radioprotective properties of 2-merkaptobenztyazol on proliferating cell culture test system.
Materials and methods: cytogenetic, cytological, statistics.
Results: Analysis of cytogenetic effects of 2-merkaptobenztiazol by frequency aberrations criteria and aberrant cells damage criteria showed that upon entire investigated range of reagent concentrations the frequency of aberrations was not significantly higher than the value of this indicator in control, but there was a tendency of reduction of mitotic activity of root meristem cells. Upon the incubation of inoculated cell line L929 with 2-merkaptobenztiazolom in the concentration range 0.03–3.00 mg/ml statistically significant change (r<0,05) of cell population density in monolayer cultures was found. At the same time for all the applied reagent concentrations stimulation of mitotic activity in the terminal (5 days) stage of cultivation was observed. Exposure of cells by gamma quanta of 60Co in doses of 1, 5 and 10 Gy led to dose-dependant morphological changes in cell culture. Exposure of cells in the presence of 2-merkaptobenztiaz significantly reduced the negative impact of radiation on cell viability parameters in culture
Conclusions. Quantifying radioprotective properties of 2-merkaptobenztyazol in the test system – cell culture L929 – showed that the highest rates of protection factor (0, 31 - 0.36) reagent had at a concentration of 3 mg/ml under the irradiation at a 1 Gy dose. However, dose reduction factor (DRF) calculated by LD50 for concentrations of 0.03 and 0.30 mg/ml had a value of 1.5 and 1.8 respectively, and the concentration of 3.00 mg/ml DRF was the maximum – 4. The literature study and the results of our own research showed that 2 merkaptobenztiazol can be considered to be reagent with radioprotective properties for cells in vitro.
Key words: frequency of chromosomal aberrations, mitosis, radioprotectors, ionizing radiation, cell culture, proliferation, apoptosis.
Problems of radiation medicine and radiobiology. 2015;20:510-525.
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