Problems of Radiation Medicine and Radiobiology

SOMATIC CHROMOSOMAL MUTAGENESIS IN PRIORITY GROUPS OF UKRAINE POPULATION IN REMOTE TERMS FOLLOWING THE CHORNOBYL ACCIDENT
[Article in Ukrainian]
S. S. Dibskiy, Ye. B. Dibskaya, M. A. Pilinskaya
SI “National Research Centrе for Radiation Medicine, National Academy of Medical Science of Ukraine”, Kyiv

For detection of late untargeted cytogenetic effects (chromosome instability) in remote terms following Сhornobyl accident high priority groups with different intensity of radiation exposure had been voluntary observed. Two-termed (during 48 and 144 hours) cultivation of peripheral blood lymphocytes and modified G2-bleomycin sensitivity assay were used. In children born to irradiated parents increased frequency of chromatid breaks in longterm cultures confirmed expression of delayed chromosome instability in consequent mitosis; appearance of stable aberrations may testify about transmissible chromosome instability. Among unexposed donors, liquidators and shelter’s personnel no more than 41.6% persons hypersensitive to testing bleomycin exposure had been identified that can be considered as genetically caused phenomenon. Among patients recovered from acute radiation sickness ~58% persons expressed hidden chromosome instability that confirmed possibility of modification of inherited chromosome susceptibility to mutagens by high doses of ionizing radiation.

Key words: ionizing radiation, human peripheral blood lymphocytes, chromosome aberrations, genome instability, Chornobyl accident.

Probl. Radiac. Med. Radiobiol. 2011. Issue 16. P. 188–196.