National Academy of Medical Sciences of Ukraine
State Institution "The National Research Center for Radiation Medicine"


ISSN 2313-4607 (Online)
ISSN 2304-8336 (Print)

Problems of Radiation Medicine and Radiobiology

  
 

   

╬. D. Pochapinskyi, G. Yţ. Lavrenchuk, N. P. Atamaniuk, A. V. Chernyshov

State Institution źNational Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine╗, 53 Yuriia Illienka Str., Kyiv, 04050, Ukraine

SELECTION AND TESTING OF EXPERIMENTAL MODELS OF NORMAL AND MALIGNANT HUMAN CELLS IN VITRO AND EVALUATION OF THEIR SENSITIVITY RANGE TO THE NEUTRON/CAPTURE AND PHOTON-CAPTURE AGENTS AND PHOTOSENSITIZERS

Objective: to investigate the structural and morpho-functional changes in test systems of malignant (A-549 cell line) and normal (fibroblasts of the 6th passage) human cells during incubation with gadolinium-containing photon-capture agent źDotavist╗ and photosensitizer źFotolon╗.
Methods. The passaged (continuously interweaved) cell culture technique on normal human fibroblasts and malignant human cells; cytological, biophysical, statistical methods.
Results. The cytotoxic properties of źDotavist╗ gadolinium-containing photon-capturing agent and źPhotolon╗ photosensitizer in a wide range of concentrations (5, 10, 25, 50, 100 and 200 μl/ml) were studied by the morphofunctional characteristics (growth kinetics, proliferative and mitotic activity, presence of atypical cells) in the in vitro test systems of malignant (non-small cell lung cancer cell line A-549) and normal (6th passage fibroblasts) human cells. It was found that the cytotoxic properties of źDotavist╗ in test systems of malignant and normal cells are expressed under its administration in high concentrations (100 and 200 μl/ml). During incubation with źPhotolon╗ photosensitizer the cytotoxic effect on malignant cells was determined at the lowest concentrations (5 and 10 μl/ml). Photosensitizer administration in the increasing concentrations has lead to genotoxic effects. Cytotoxic effect of photosensitizer on the normal human fibroblasts was evident in the 5-200 μl/ml concentration range. There was a moderate decrease in mitotic activity along with increasing concentration. Genotoxic properties of photosensitizer were evident at 25 μl/ml concentration and above.
Conclusion. Study results of the effectiveness of neutron-capture and photon-capture technologies by the sensitivity assay in the in vitro test systems of human malignant cells (non-small cell lung cancer cell line A-549) and normal cells (transplantable human fibroblast culture, the 6th passage) to the gadolinium-containing photon-capture źDotavist╗ agent and źPhotolon╗ photosensitizer in different concentrations provide the basis for pre-clinical stage of evaluating the effectiveness of medications used in binary technologies.
Key words: culture of human malignant cells, culture of human fibroblasts, neutron-capture agent, photon-capture agent, photosensitizer, proliferation, mitotic index.

Problems of Radiation Medicine and Radiobiology.
021;26:260-272. doi: 10.33145/2304-8336-2021-26-260-272

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