Problems of Radiation Medicine and Radiobiology

D. I. Bilko, I. Z. Russu, N. M. Bilko

National University of Kyiv-Mohyla Academy, 2, Hryhoriia Skovorody St., Kyiv, 04070, Ukraine

ASSESSMENT OF RADIOPROTECTIVE ACTION OF BASIDIOMYCOTIC MELANIN PIGMENTS ON THE HEMATOPOIETIC SYSTEM OF Balb/C MICE UNDER EXPOSURE TO IONIZING RADIATION IN SUBLETHAL DOSE

Objective. Assessment of radioprotective action of basidiomycotic melanin pigments on hematopoietic stem and progenitor bone marrow cells of Balb/C mice in case of exposure to ionizing radiation in sublethal dose.
Materials and methods. Using original method of cultivation in gel diffusion chambers in vivo of bone marrow cells of Balb/C mice we investigated the colony-forming efficiency of hematopoietic progenitor cells of the animals, which were exposed to ionizing radiation action in sublethal dose, in case of treatment with melanin pigments solution of basidiomycotic fungi as radioprotector.
Results and conclusions. Investigation of functional activity of bone marrow progenitor cells of Balb/C mice allowed assessing their hematopoiesis state in case of ionizing radiation action, as well as in case of previous treatment of the animals with the solution of melanin pigments as radioprotector. It was determined that under the influence of ionizing radiation the colony-forming activity of mice bone marrow has decreased comparing to control. Solution of melanin pigments was able to enhance the functional activity of bone marrow of irradiated animals. Obtained results of radioprotective action of basidiomycotic melanin pigments solution on irradiated stem cells and their descendants (progenitor cells) may become the evidence for development of the protective means for human organism from the injuring action of ionizing radiation.
Key words: hematopoiesis, ionizing radiation, progenitor cells, melanins, cell culture.

Problems of Radiation Medicine and Radiobiology.
2019;24:210-219. doi: 10.33145/2304-8336-2019-24-210-219

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