Problems of Radiation Medicine and Radiobiology

O. A. Boiko¹, H. Yo. Lavrenchuk¹, A. I. Lypska², V. V. Talko¹, V. S. Asmolkov³

¹State Institution «National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine», Melnykova str., 53, Kyiv, 04050, Ukraine
²Institute for Nuclear Research of the National Academy of Sciences of Ukraine, Prospect Nauky, 47, 03680, Kyiv, Ukraine
³Institute of Biochemistry named after O. V. Palladin of the National Academy of Sciences of Ukraine, Leontovycha str., 9, 01601, Kyiv, Ukraine

Features of morfological changes in primary thyroid gland CTLL cultures of rats descendants prenatally exposed by radioisotopes of iodine-131

Objective: to investigate morphological changes in the primary thyroid cell culture of rat infants whose parents were prenatally exposed by radioisotope iodine-131.
Materials and methods: obtaining and culturing of thyroid tissue primary cell cultures of newborn rats, cytological (receipt and analysis of cell cultures agents for optical microscopy), biophysical (flow-cytometry), statistics.
Results. It was shown that cells in thyroid primary culture of offspring rats prenatally exposed by radioisotopes of iodine-131 signs of destructive-degenerative changes were observed mostly when animals of both sexes were irradiated. Increased number of two- and three nuclear cells and induction of ring-like cells is an evidence of significant genotoxic violation and points to the genome instability in offspring of animals exposed by radioisotope iodine-131.
Conclusions. Analysis and quantitative morphological parameters of cells in thyroid primary culture of newborn rats whose parents were exposed prenatally by radioisotopes of iodine-131 showed that upon exposure to radiation thyroid undergoes destructive changes at the cellular level and, even in the second generation of offspring, leads to disruption of its functions.
Key words: radioisotope iodine-131, thyroid cell culture of rat, mitosis, proliferation, apoptosis.

Problems of radiation medicine and radiobiology. 2017;22:172–183.