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Nurishchenko N. E.
Effect of ultrasound on the interleukin content in blood of rats with experimental inflammation
The aim was to determine the effectiveness of anti-inflammatory action of ultrasound by the interleukin-6, interleukin-8 and tumor necrosis factor alpha content in the blood of rats with experimental inflammation.
Materials and methods: the study was performed using conventional models of inflammation - carrageenan-induced edema of the limbs of rats. Content interleukins IL-6 and IL-8 were determined by enzyme immunoassay (ELISA) according to a standard protocol Immunoassay kit IL-10, Biosourse (USA). The content of TNF-? was determined by cytotoxic effect on sensitive mouse fibroblast line L-929.
Results and discussion: it was shown that in the group of rats with carrageenan-induced edema contents of IL-6 increase in 2.8 times, IL-8 – in 5.6 times, and TNF-? – in 3 times compared to the control. Course influence of ultrasound contributed to reduction of the studied parameters. The content of IL-6 decreased in 1.7 times, IL-8 and TNF-? – in 1.6 times in comparison with inflammation.
Key words: ultrasound, inflammation, interleukin-6, interleukin-8, tumor necrosis factor alpha.
Problems of radiation medicine and radiobiology. 2015;20:526-532.
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