National Academy of Medical Sciences of Ukraine
State Institution "The National Research Center for Radiation Medicine"


ISSN 2313-4607 (Online)
ISSN 2304-8336 (Print)

Problems of Radiation Medicine and Radiobiology

  
 

   

V. V. Zhirnov1, I. N. Iakovenko1, V. M. Voitsitskiy2, S. V. Khyzhnyak2, . G. Zubrikova-Chugainova3,
V. A. Gorobetz3

1Institute of Bioorganic Chemistry & Petrochemistry of National Academy of Sciences of Ukraine., 1 Murmanska St., Kyiv, 02660, Ukraine
2National University of Life and Environmental Sciences of Ukraine, 15 Heroyiv Oborony St., Kyiv, 03041, Ukraine
3Public institution "Institute of Heart" of the Ministry of Health of Ukraine, 5A Bratyslavska St., Kyiv, 02660, Ukraine

Zeta potential response of human erythrocyte membranes to the modulators of Gardos channel activity under low rate β-radiation

Objective. Study of human erythrocyte DP response under modification by activators and blockers of the functional state of Ca2+-dependent K+ channels under low rate β-radiation.
Materials and methods. Erythrocytes were isolated from the donor blood. The zeta potential was computed from the value of the cell electrophoretic mobility. The investigated drugs preliminary introduced in cellular suspensions, and then aliquote of 90Sr(NO3)2 solution to get the final activity concentration of 44.4 kBql-1.
Results. The radioisotope radiation of 90Sr/90Y (RR, 15 mGyh-1) increases an absolute value of erythrocyte membranes DP (DPab), and its action is reversible. It specifies the effect is mediated by nonionizing part of the RR. Dibutyril-cAMP dose-independent increases DPab of erythrocyte membranes in the concentration range of 1100 μ, but RR does not amplify this effect. Anapriline increases dose-independent DPab in concentrations 10 and 100 m. The effect of maximal concentration of anapriline (100 μ) decreases by RR. Clotrimazol increases DPab of erythrocyte membranes in the concentration range of 0,110 μ relatively control, while its maximal concentration decreases, and the minimal level does not reliably influence on this index The action of lotrimazol on DP in concentrations of 10100 μ is abolished by RR, and is not changed in the range of 0,11,0 μ. Nitrendipine raises DPab of erythrocyte membranes in all of range of concentrations, and RR amplifies the effect of the drug.
Conclusions. 1. There is a threshold of the biological action on cells for the ionizing component of radioisotope radiation determined by effectivity of operation their antioxidant system.
2. At dose rates below a threshold, the action of ionizing radiation is mediated by its nonionizing component, and is reversible, and therefore is determined only in the field of radiation.


Key words: Gardos channel, zeta potential, β-radiation, strontium-90, human erythrocytes, biomembranes.

Problems of radiation medicine and radiobiology. 2015;20:490-499.



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